Faculty of Chemistry and Biology / Department of Biology
3 (1)
DESCRIPTION
The Laboratory of Molecular Microbiology, directed by Dr. Carlos Vera and Dr. Felipe Arenas, is dedicated to the identification and characterization of metal- and metalloid-resistant bacteria, as well as the synthesis of non-toxic insoluble metals through biotransformation processes. In addition, it focuses on the identification, cloning, expression and purification of extremophilic enzymes for their application in the biotechnological industry.
The laboratory is also specialized in biocatalysis and biotransformations, centering on the use of glycosidases for revaluing residues. Its research includes the study, development and optimization of the synthesis of high added-value compounds from carbohydrates, using biocatalyzer and reaction engineering strategies. Some of the laboratory’s active research lines are galacto-oligosaccharide and alkyl-galactoside synthesis, both with prebiotic applications and applications in the production of non-toxic and biodegradable tensoactives.
LÍNEA DE INVESTIGACIÓN
Enzymatic Biocatalysis
Biotransformations
Process simulation and optimization
Nanostructures biosynthesis using metalloid- and metal-reducing flavoproteins
Identification and production of extremophiles’ enzymes
EQUIPMENT
Total surface of 95 m2 divided into a room for cultures, microorganism handling and storage, a washing/sterilization room, and laboratory tables for the simultaneous work of 12-15 students
2 Coy anaerobic chambers (25 and 37 °C)
Microcentrifuges, refrigerated centrifuges
-20 and -80 ° freezers/ultrafreezers, refrigerators, refrigerated cabinets
Laminar flow hoods
DNA and electrophoresis full equipment (vertical and horizontal)
Fraction collectors
Thermoregulated baths
UV transilluminator
High resolution photographic camera
Culture incubators and sterilizing ovens
Gel drier
High voltage and conventional power supplies
Electroporator
Amicon protein concentration device
4 orbital agitators
Sterilizers
Gradient thermocyclers
HPLC UV- detector, spectophotometer and 2 ELISA TECAN Infinite M200 PRO readers (aerobic and anaerobic)
Minifors 2 (Infors, Switzerland) biorreactor with 6 L capacity
Analytical and conventional scales
Optical microscope
Ultrasonic disruptor
Conventional and heated magnetic agitator
Vortex
STAFF
Dr. Felipe Arenas head of laboratory
Dr. Carlos Vera head of laboratory
Fernanda Contreras PhD candidate
Katherine Rivero PhD candidate
Nicole Neira Asistente de investigación
Andrea San Martin PhD candidate
Inaida Rivero PhD candidate
Jorge Torres Undergraduate thesis student
Rodrigo Valenzuela Undergraduate thesis student
CONTACT INFORMATION
Correo de contacto: felipe.arenass@usach.cl – carlos.vera.v@usach.cl Teléfono de contacto: +56 227181118
ADDRESS Casa Central, Primer piso Av. Libertador Bernardo O’Higgins 3363, Estación Central, Santiago de Chile.